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The results showed a high efficiency of the assay developed, ranging from 93% to 101% with r2-values of > 0.99.
Under the optimum conditions, the fluorescence recovery efficiency of the assay is proportional to the concentration of clenbuterol.
The efficiency of the assay was estimated to be 92.41%, with 100% specificity, and no cross reactivity was detected with any other species.
The speed of the fractionation is critical since the efficiency of the assay decreases proportionally with time.
Also, the presence of multiple mutations and point deletions may alter the efficiency of the assay, possibly the reason for low performance in TP53 assays [16], [43].
A ten-fold dilution series of genomic DNA showed a difference of 3.32 3.6 Ct (cycle threshold) per dilution and a standard curve with a slope of −3.24, both of which reflected maximum efficiency of the assay system (Figure S2).
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The KM for most protein kinases is approximately 50 micromolar, raising the possibility that the enzymatic efficiency of the array assays at 33 nM ATP may not be optimal.
These advantages include the ability to handle small sample sizes with minimal reagents, the potential applications in field use or point-of-care (POC) usage, the possibility of integrating with biosensors or sample-pre-treatment modules, which increases the efficiency of the assays and reduces cross-contamination, decrease in the cost of test and short assay time [58, 59].
The average PCR efficiency of the assays was 0.92 for F. verticillioides and 0.98 for F. proliferatum.
Sequence polymorphism among alleles in the different families influences the efficiency of primer binding and therefore the overall efficiency of the assays.
For the SMART-MSP assays the standard dilution series were, in addition, used to assess the quantitative accuracy and the PCR efficiency of the assays using the LightCycler 480 Software Version 1.5.
More suggestions(15)
efficiency of the transmission
efficiency of the product
efficiency of the motor
efficiency of the installation
efficiency of the transfer
efficiency of the plant
efficiency of the method
efficiency of the conversion
efficiency of the transport
efficiency of the device
efficiency of the source
efficiency of the intervention
efficiency of the network
efficiency of the laser
efficiency of the program
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