Sentence examples for efficiency of the amplification from inspiring English sources
The efficiency of the amplification strategy based on Au nanoparticle is discussed.
Efficiency of the amplification was verified by the analysis of standard curves.
TaqMan probe and primer sets were designed to avoid polymorphisms, while maximizing the efficiency of the amplification.
Nested PCR was performed to enhance the efficiency of the amplification in order to increase the amount of DNA available for cloning.
Each product was checked for its specificity in melting curve analyses and efficiency of the amplification was verified with standard curves for every gene.
To assess the efficiency of the amplification reactions, standard curves for every primer pair and cDNA were generated from six-fold serial dilutions in duplicate, using the iQ5 software.
Primer and probe concentrations were optimized and to determine the efficiency of the amplifications, dilution standard curves were made for each set of primers and probe (data not shown).
Efficiencies of the amplification reactions were calculated with a typical sample analysing the slope of the regression line of a 10-fold dilution series of cDNA (log10) versus CT: Efficiency =10-1/slope.
Validation experiments were performed to test the efficiency of the target amplification and the efficiency of the reference amplification.
The variation, linearity and efficiencies of the amplifications were calculated according to the mathematical algorithms stipulated by Pfaffl [ 40].
The inverse and complementary sequences of primers (13 bases) contained in the library structure were expected to interfere in the efficiency of the polymerase amplification.
