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Although the variation in coverage could be partially due to experimental bias (ie differential cloning efficiency of sequences [ 35]) it is also likely to reflect in vivo abundances.
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The project is becoming possible as the speed and efficiency of sequencing increase dramatically, and the once-prohibitive costs drop from millions of dollars for a genome two years ago to under $10,000 today.
In order to increase the clinical and economic efficiency of sequencing, geneticists may analyze only those portions of the genome that have a high likelihood based on prior studies of being associated with the particular symptoms of the newborn.
Extrapolation of the curve indicates that efficiency of sequence recovery would approach zero at ~20% observed sequence divergence.
The efficiency of sequence-specific hybridization is dependent on the properties of the probe and target sequences [ 18].
Therefore, the efficiency of sequence-based typing of L. pneumophila can be improved by direct amplification and sequencing of DNA extracted from uncultured respiratory samples (22 ).
Efficiency of sequence recovery declines with the increase of the number of interspecific SNPs in the 25b interval tiled by the reference oligonucleotides.
We suspect the differences between qRT-PCR and RNA-seq arise from the nature of RNA preparation and from the manner and efficiency of sequence detection and amplification.
Because of the rapid increase in the speed and efficiency of sequencing technologies, it has long been speculated that routine WGS was a near-future inevitability.
We demonstrate the efficiency of sequencing gene rich regions from barley chromosome 3H, with special reference to syntenic relationships with rice chromosome 1.
When sufficiently effective on a sample, this approach can increase the cost efficiency of sequencing plant genomes as well as prokaryotes living in or on plant tissues.
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