Sentence examples for efficiency of mutation from inspiring English sources

Alternatively, the efficiency of mutation analysis could be increased by modifications in DNA template production from large populations.

This would greatly facilitate progress in the study and breeding of soybean and other polyploids in which the efficiency of mutation breeding might otherwise be low.

Similar results were obtained with sgRNA, as exhibited by a significantly low efficiency of mutation with 1 ng/µL sgRNA (46.4±6.6%) as compared to 10 and 25 ng/µL (96.5±1.3% and 95.7±2.5%, respectively) (Fig. 3D).

Although applying our version of GINI to simultaneous analysis of multiple cell lines of the same tumor type could dramatically improve the efficiency of mutation identifications, false positives caused by cell line-specific variability in the efficiency of NMD degradation of transcripts representing the natural substrate of NMD put limit on GINI efficiency.

With regard to SHM, we show that the amount of SMUG1 activity in a UNG‐deficient hypermutating cell is not sufficient to excise uracil from many of the AID‐generated U G lesions before the replication fork passes but reveal that even a small contribution from UNG or SMUG1 to uracil excision (while not providing the sole route of entry into phase 2) impacts on the efficiency of mutations at A Ts.

Our modified protocol significantly increased the efficiency of single mutation and also allowed facile large single insertions, deletions/truncations and multiple mutations in a single experiment, an option incompatible with the standard QuikChange™.

For CHEK2 amplicons harboring SNPs with a frequency ≥1% in either the Single Nucleotide Polymorphism Database (dbSNP) [ 33] or initial amplicon testing, we applied a simultaneous mutation scanning and genotyping approach using HRM curve analysis to improve the sensitivity and efficiency of the mutation screening [ 34].

For MRN amplicons harboring a SNP s) with frequency ≥1% in either dbSNP or initial amplicon testing, we applied a simultaneous mutation scanning and genotyping approach using HRM analysis to improve the sensitivity and the efficiency of the mutation screening, as described previously [ 24, 25].

If k j is equal to the old one, then select a new positive integer again until they are different; the efficiency of the mutation could be greatly improved using the method.

To test the efficiency of plets1 mutation, we measured the DA content in the plets1 males raised at 25°C (18.7±2 ng/mg mass, s.e.m. N = 5) and at 31°C (8.6±1 ng/mg mass, s.e.m. N = 6) respectively.

Taken together, these results show that the efficiency of heritable mutation is dependent on the proper distance as well as orientations between the two sgRNA targets.