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The spectral shape as well as dynamic behavior of the emission remains unchanged upon coupling with the nanospheres; therefore, we attribute the observed enhancement as being due to enhanced efficiency of light collection from molecules in the vicinity of the silica nanoparticles.
Third, the efficiency of light collection by the detection system could be improved by clever geometries.
It can be used to check the efficiency of light collection by the laser Doppler probes.
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These include considerations of key performance parameters, such as the efficiency of scintillation light collection, restrictions placed on the use of materials to control the inherent radioactivity levels, attainment of high vacuum levels and chemical contamination control.
Because the experimental method does not descan the emitted Eu3+ luminescence, there is no loss of efficiency in light collection due to a pixel dwell time that is less than the luminescence lifetime.
Longer carbon chains produced bigger spacing, which dramatically affected the efficiency of light emission.
The transverse resolution benefit of using a sub-Airy disk confocal aperture comes at the price of reduced light collection efficiency, as indicated by the plots on the right column, which could be a major limitation when the number of photons that can be delivered to the sample is limited.
Optics with large surface roughness may suffer from scattering issues, decreasing the efficiency of the contrast and light collection [26].
The overall intensity I feat, meas = f (x)·(I feat + I back ) is affected by the function f(x) which accounts for spatial variations of the fluorescence excitation and the light collection efficiency of the microscope system (e.g. due to vignetting).
A fluorescence standard (Rhodamine B, 1 μ M in water) was used to calibrate the intensity of the tissue fluorescence for changes in excitation intensity and light collection efficiency of the optical system.
The light collection efficiency of a well-designed microscope is greatly enhanced over other optical arrangements.
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