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However, the efficiency of isolation of microorganisms that degrade solid forms of biodegradable plastic is low.
It is likely that the chromatin states of the telomeric structures, promoters, and genes, lead to differences in the efficiency of isolation of chromatinized DNA, prior to the shearing step or during the reversal of crosslinking.
In catfish, similar efficiency of isolation of microsatellites from cDNA was reported [ 18].
There could have been systematic change in recent years in the efficiency of isolation, either through reduced specimen delay or improved culture media or culture conditions (eg, atmosphere).
An isolation method for mouse germline stem cells using antibodies to Fragilis, which is a known transmembrane protein (60), for antibody-assisted magnetic-bead sorting has been established (61) and may offer greater efficiency of isolation.
In addition, a simplified protocol for the isolation and the growth in vitro of TILs has been set up to improve both the efficiency of isolation of these T lymphocytes and their anti-tumor potential for ACT studies [ 26, 36].
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After DNA isolation, the efficiency of the isolation procedure was determined by IS 900 qPCR in two independent experiments.
This pre-isolation step improved the efficiency of DNA isolation in comparison to the results obtained with the use of commercial DNA extraction kit only (Genomic Mini, A&A Biotechnology, Poland) (data not shown).
The efficiency of the isolation system is investigated by analyzing the effects of various parameters such as; (i) isolation period, (ii) tank aspect ratio and (iii) coefficient of friction.
We developed an internal control which was efficient to avoid false negative results due to decreases in the efficiency of DNA isolation and/or amplification.
Also, the efficiency of gene isolation after reporter expression-mediated trapping should be optimized in such a selected sub-population.
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