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The rate and efficiency of internalization depends on the type of target and the choice of cytotoxin.
We wanted to see how the voltage used affects the efficiency of internalization of our optimized ω-Cy3b sample.
On the other hand, low temperature (4°C) affected negatively the cell uptake of crotamine, since the efficiency of internalization could be drastically reduced showing the retention of the crotamine on the cell surface [ 17].
We picked up a time point of 10 min to examine the efficiency of internalization as we used 10 min as an end point to compare the different activation of B cells when encountering NP-TGT sensors providing low-, middle- or high-forces in our original and revised manuscript.
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Mutations in the beta2AR tail that ablate NSF binding reduce the efficiency of receptor internalization upon agonist stimulation.
Human lung alveolar epithelial A549 line and normal lung fibroblast cell line (MRC-5) cultured in each PDMS/glass hybrid channel are used to quantitate the efficiency of cellular internalization of NPs at different sizes and surface properties.
The cellular concentration of muniscins regulates clathrin-coated structure organization and dynamics and, thereby, the efficiency of cargo internalization.
Radicicol did not affect the efficiency of luciferase internalization, indicating that decreased enzymatic activity in the cytosol was specifically due to Hsp90 inhibition (Supplementary Figure 6).
The preincubation of the cells with dextran sulfate could further enhance the efficiency of TN122 internalization in a dose-dependent manner as determined by increased amounts of TN122 detectable in cellular lysates prepared from transduced Kasumi-1 cells).
In this context, Faf deubiquitinates Liquid facets (Lqf), the Drosophila Epsin homolog, to increase the activity of Lqf, which in turn enhances the efficiency of Delta internalization (Chen et al. 2002).
Fleire et al. can only examine and quantify the efficiency of the internalization of antigen at an end point as early as 2 hrs after placing the B cells on PLBs containing different densities of antigen (20, 100, and 200 molec/µm) (Science, 2006, 312 738-41).
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