Exact(21)
The high efficiency of expression knockdown was reproducible with more than 70% of the F0 individuals showed sufficient gene expression suppression (<30% of the controls' levels).
Taken together these observations suggest that natural selection is directly acting on gene sequences to maximize the efficiency of expression and that regional variables may, eventually, concur to this action.
We further assessed the efficiency of expression and the solubility of three ADHs in E. coli.
Transient knockdown efficiency of expression or upregulation of proteins was achieved as shown by Western Blot.
Real-time RT-PCR analyses were executed for evaluating the efficiency of expression.
However, the efficiency of expression of transferred RNA may also profoundly influence the extent of the expected phenotypic changes.
Similar(39)
In agreement with the different efficiencies of expression observed for the GFP marker, the FIX levels supported by IDLVs were up to 15-fold lower than those supported by the cognate ICLVs.
In our study, we found widely differing levels of efficiency of TIC expression relative to expression of the 5' gene.
To assess the efficiency of transgene expression, GFP-expressing AAVs were also generated.
We, thus, observed that the efficiency of stable expression is higher when there is more pT2MIK-eGFP expression plasmid than transposase-supplier helper plasmid.
Linear DNA traverses nucleopores efficiently [ 52] and may therefore improve delivery efficiency of RNAi expression cassettes.
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