Sentence examples for efficiency of editing from inspiring English sources

In view of the high efficiency of editing of HBB in the previous study (Xie et al., 2014), more efficient gRNAs for HBB gene than the ones tested in this study might exist, and the whole targeting strategy could be more cautiously optimized.

The efficiency of editing, however, still remains variable from locus to locus, likely owing to differences in sgRNA efficiency and possibly differences in locus competency for HDR.

As shown for DNA nicks, DSB repair using ssDNA and dsDNA templates might involve different repair machineries resulting in different efficiency of editing (Davis and Maizels 2014).

We found no correlation between the amount of TDP-43 expression in M17 cells and the efficiency of editing in the GluR2 transcript (Supplementary Fig S20), indicating that dsRNA accumulation is not due to decreased ADAR2 function.

However, as both the biocuration team and the amount of curated data increased substantially in size, it was necessary to develop an application that would expedite the curation process, centralize all core curation activities, eliminate the bottleneck of spreadsheet integration and enhance the efficiency of editing and QC review.

To analyse the efficiency of editing, the heights of the peaks at an editing location within the trace files were measured and compared with one another as illustrated in Fig. 2. The editing efficiency was calculated using the formula (Nakae et al. 2008): Polymerase chain reaction products of the ndhB and one ndhF fragment were sequenced.

Efforts to improve Cas9 specificity, like the development of RNA-guided FokI-nucleases (RFNs), usually come at the cost of editing efficiency and/or genome targetability.

Therefore, our results suggest that a decay in editing efficiency precedes the loss of editing sites, as proposed by Schields and Wolfe [ 10].

For example, it has been shown experimentally that internal loops may effectively be equivalent to helix termini in terms of editing efficiency [ 42].

CRISPR/Cas9 editing efficiency in C. elegans depends on the type of edit, location of the DNA double-stranded break, and whether a selection scheme is used; Table 4 denotes the current range of editing efficiencies with current approaches in C. elegans.

According to the edited proportion of each nucleotide in sequence reads with a C-to-U or U-to-C conversion after mapping to the corresponding DNA template over the total reads in that nucleotide position, the efficiency of RNA editing for each edit was further classified into five groups.