Exact(11)
HeLa nuclear extracts were immunodepleted of either PC4 or CK2 and the depleted extracts analyzed by Western Blot to assess the efficiency of depletion (Supplemental Figure S1A).
For efficiency of depletion, see Table S1.
Efficiency of depletion was analyzed by FACS using anti-pan TCR γδ antibody GL3 [25].
Efficiency of depletion was monitored by flow cytometric analysis of GFP positive cells.
Efficiency of depletion was analyzed by flow cytometry of lymphoid organs, staining for CD4, CD25 and FoxP3.
The efficiency of depletion is affected by several factors, including the efficiency of the shRNA/miRNA, the mRNA levels, the half-life of the mRNA and protein of interest, and the cell line used.
Similar(49)
We observed degradation of OPA1 in cells depleted of prohibitins, which correlated with the efficiency of prohibitins depletion.
We analyzed the efficiency of TPX2 depletion by immunoblot.
The efficiency of cell depletion was >95% as measured by FACS using CD3, CD4, and CD8 Abs.
In order to maximize the efficiency of the depletion, in the light of TACC3 being already present in prophase, oocytes injected with the siTACC3 were maintained in prophase for a prolonged period of time (30 hours) before meiotic maturation was allowed to proceed.
Additionally, the efficiency of Treg depletion was measured by monoclonal antibody staining of isolated splenocytes with anti-CD25 APC-conjugated (clone PC61), anti-CD4-peridinin chlorophyll protein (PerCP) (clone L3T4; BD Bioscience) and anti-FoxP3 PE-conjugated (clone FJK-16S) antibodies (both BD Biosciences).
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