However, no 6.5-kb DNA band was detected under the cultivation conditions used in this study, indicating that the efficiency of excision by homologous recombination between the IS elements and/or the stability of the circular elements of strains TCE1 and Y51 might be different.
These mutant RTs lead to resistance by increasing the efficiency of excision of the chain-terminating AZT monophosphate from the terminus of the nascent DNA chain by ATP-mediated phosphorolysis.
We have used the relative neo gene copy number information from RT-PCR genotyping as a proxy for determining the efficiency of excision of the Lo (c-fos-3'UTR and neo) portion of the Ctgf Lo-Hi allele.
However, AAG is not processive on ssDNA because of the low efficiency of excision.
Efficiency of excision in the ZP3-Cre line was tested by the ZAP CAG-Bgeo/ALPP reporter.
This corresponds to an efficiency of excision of 0.95 under these conditions.
Liu et al. [ 20] found that the Arabidopsis xpd mutant lines were characterized by growth defects, decreased UV resistance, and limited efficiency in excision of UV photoproducts.
The ZP3-Cre 3Mrt line has a high efficiency of excision (∼98%), with activity restricted to oocytes initiated at the primordial follicle stage (Fig. S3A).
This difference between ssDNA and dsDNA contexts could be explained by differences in searching ability or by the decreased efficiency of excision in the single-strand context.
In contrast, the efficiency of excision of the 8-oxoG lesion when an AP site is position +1 is severely retarded compared to an 8-oxoG lesion present in isolation (Fig. 4B and C).
