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In agreement with the different efficiencies of expression observed for the GFP marker, the FIX levels supported by IDLVs were up to 15-fold lower than those supported by the cognate ICLVs.
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The high efficiency of expression knockdown was reproducible with more than 70% of the F0 individuals showed sufficient gene expression suppression (<30% of the controls' levels).
Taken together these observations suggest that natural selection is directly acting on gene sequences to maximize the efficiency of expression and that regional variables may, eventually, concur to this action.
We further assessed the efficiency of expression and the solubility of three ADHs in E. coli.
Transient knockdown efficiency of expression or upregulation of proteins was achieved as shown by Western Blot.
Real-time RT-PCR analyses were executed for evaluating the efficiency of expression.
However, the efficiency of expression of transferred RNA may also profoundly influence the extent of the expected phenotypic changes.
For a target gene of interest, many siRNA molecules may be designed, whereas their efficiency of expression inhibition often varies.
This confirms that the difference in the efficiency of expression of the second cistron is solely the result of differences in the IRES AUG initiation codon flanking sequences.
All repeated experiments revealed that, despite the comparable efficiency of expression of both nucleases, the yield of nuclease activity was significantly higher for CAN2.
While the bursal DT40 cell line has multiple valuable features for research [16], the transfection efficiency of expression plasmids is usually very low.
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CEO of Professional Science Editing for Scientists @ prosciediting.com