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Therefore, animal models in general are not useful for efficacy validation due to species specificity of gene sequence.
The generalizability of this study is limited, because extrapolating conclusions from experiments on animal models to humans requires safety and efficacy validation.
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In conclusion, further studies on how Nano-RBCs improve STS efficacy require experimental validation.
Specifically, the unexpected association of PDGFR phosphorylation in peripheral blood leukocytes with taxane efficacy requires prospective validation and these studies are planned in the phase III setting where larger sampling is feasible; for example, a sample size of 180 is required to confirm a HR of 2.3 for PFS with Pr(Decr-pPDGFR) >0.5 with a power of 0.82.
What will validation validate?
We used previously validated survey tools on illness beliefs and medication adherence self-efficacy, but the validation was not done in an Afro-Caribbean population and these scales may not accurately reflect true perspectives of our target population [17, 18].
Model cross-validation efficacy (also measured using kappa and percentage agreement statistics) must be performed manually (in our case, using SPSS, version 19.0).
As we assume that physicians rate their personal importance of teaching very differently and that both constructs share a great overlap, we chose to assess self-efficacy for this validation study.
Modern medical pharmacotherapy is based on extensive testing and validation of efficacy and safety utilizing various methodologies.
The differentially expressed proteins can be targets for validation and efficacy testing of future therapies in PXE fibroblasts (targeted compound screening; see above) [ 46].
This step has to be followed by other phases and markers, sensitivity and specificity need to be tested and established after appropriate validation before efficacy can be assessed using different diagnostic methodologies.
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