Combined, these technical shortcomings affect the validity of some expression signatures and limit the efficacy of projecting expression signatures between data sets.
In order to fully characterize the efficacy of our expression system, we tested the viral titer of plko.YFP.DEST expressing genes of various sizes in 293T cells.
Western blot analysis confirmed the efficacy of ERβ expression to down modulate EGFR, Akt and ERK1/2 phosphorylation without a change in the total abundance of these proteins expression, while it resulted in cyclin D1 protein reduction (Fig. 1A).
In plants, there exists conflicting results regarding the efficacy of protein expression from T7-Pol-derived mRNAs.
The engineering of cell-specific enhancers to improve potency and the development of two-step transcriptional activation (TSTA) approaches have significantly improved the efficacy of transgene expression.
The present study, therefore, was designed to determine the efficacy of transgene expression using the GeneSwitch mifepristone-regulatable system within the context of an integrating HIV-1 vector.
The role of polymer physicochemical properties in determining efficacy of transgene expression was investigated using Quantitative Structure Activity Relationship QSARR) cheminformatics models based on Support Vector Regression (SVR) and 'building block' polymer structures.
Our aim, instead, was to test the efficacy of gene expression microarrays for detecting microbial responses to environmental change.
Small sample size and the large number of measurements required for each sample currently limit the efficacy of gene expression profiling, leading to efforts to develop new analytical methods.
