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The QTL effects were validated in backcross populations.
In this study, we used the MTT test as previously described, and all significant effects were validated by cell counting.
Predicted effects were validated by RNA-seq analysis of the three aforementioned cancer cell lines, and expression microarray analysis of SNPs in HapMap cell lines.
These effects were validated using a series of NILs which showed, on average, over 5% improvement in yield and TGW in four of the five environments tested.
The effects were validated in an embryonic kidney cell line (HEK293T) and in HLRCC-patient derived cells (UOK262) via both genetic and pharmacological inhibition.
siRNA knockdowns were used to suppress S6K1 and GLI1 expression, and the siRNA effects were validated by real-time PCR and Western blotting.
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Bold miRNAs indicate cross-platform validation which means that at least one of the treatment effects was validated (significant and >2-fold regulation) on both platforms.
Again, the normality assumption on the random effects is validated by the structure of the matrix which is almost diagonal.
These effects are validated by silicon ageing tests on structures designed in 28 nm Full Depleted Silicon on Insulator (FDSOI).
The ability of the dual-reporter assay to measure stage-specific drug effects was validated treating for 48 h mixtures of stage II and of stage V gametocyte cultures with 500 nM chloroquine, virtually inactive on mature gametocytes, or 100 nM epoxomicin, killing all sexual stages.
The effect is validated with experimental testing.
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