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However, somewhat surprisingly no, or very modest, effects on the repression of Dicer mRNA and protein levels were observed after HIF-1α and HIF-2α inhibition using siRNA treatments.
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However, the effect of the repression of histone genes on cell cycle in rice panicle under drought needs to be further elucidated.
As shown in Figure 3B, LANA was able to repress the activity of the IL-22R1 WT promoter in a dose-dependent manner, whereas it had a much lower effect on the repression of pIL22R1ΔLBS-like and minimal effect on the activity of pIL22R1-mLBS-like.
Since both strains showed similar defects, Rts1 exerts its effect on the repression of SUC2 likely through PP1.
The overexpression of TBP2 or presence of competitor DNA, alone or in combination, had little if any effect on the repression of transcription.
Thus, WNT3A did not enhance differentiation toward the mesodermal cell state in our system, but exhibited an antagonistic effect on the repression of mesodermal genes by KSR.
However, the extent and manner that ceRNAs can exert a consequential effect on the repression of targets for that miRNA is currently unclear (Broderick and Zamore, 2014).
To assess the effect of Sum1 on the repression of Ndt80 target genes, we analyzed the changes in the expression pattern of 140 Ndtargetsgethathat show late phase repression (in the WT strain, see Methods).
The effect of DBC1 on the repression of the BMAL1-luciferase activity was also detected when DBC1 was transfected in the absence of Rev-erbα, possibly due to an effect of DBC1 in endogenous Rev-erbα.
A strain deleted for the SPT3 equivalent, sptC, was similar to the acdX deletion strain in that it showed apparent effects on acetate repression using the phenotypic analysis, but there was no derepression of alcA or aldA in RT-qRCR experiments.
In order to identify the mechanisms that lead to drug-induced differentiation, we first analysed the effects on PcG repression.
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