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This study was aimed to explore the standard addition method as a means to overcome the plasma matrix effects on the quantification of cathinone and mephedrone by LC-MS/MS.
These unwanted effects on the quantification of small structures need to be thoroughly studied in a clinical relevant setting as these artifacts are not revealed by the standard NEMA image quality test [13].
Additionally, VOIs in the shape of spheres with a radius of 2 mm were placed in the center of the same organs to limit the impact of partial volume effects on the quantification and used for dosimetry (referred to as 'dynamic single').
To investigate matrix effects on the quantification, we spiked the protein-containing reaction buffer with 10 800 nmol L−1 of the analytes.
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This study is aimed to use the standard addition method to overcome the plasma matrix effect on the quantification of cathinone and mephedrone by LC-MS/MS.
In this paper, we demonstrated that the choice of a gene model has an effect on the quantification results.
We employed an external calibration curve for quantification of 8-oxodG in these studies, to investigate whether the presence of matrix has any effect on the quantification of samples.
We showed that variation of pH from 3 to 9 and increases in osmolarity up to 6-fold had no effect on the quantification of the metabolites or on multivariate data analysis.
This so-called 'isotope effect' obviously complicates peptide matching, but has been shown to have little effect on the quantification accuracy, as long as the abundances of the deuterated peptides are measured along their whole elution profiles, rather than in one particular scan (Boersema et al. 2009; Ji and Li 2005).
Artificial variation of pH even with these extreme changes in the range from 3too 9 followed by buffering of the samples (pH range 6.62 to 7.64) resulted in relatively mild effects on the single metabolite quantification except for urea).
In this study, different combinations of the compensation methods are applied during OSEM reconstruction and the effect on the 177Lu quantification accuracy is studied in an anthropomorphic torso phantom.
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