Use of shRNA directed against Skp2 resulted in increased expression of the p27WT, p27T198V or p27T198E proteins but produced only minor effects on the half-life of p27T198A or p27T198G (Figure 2C).
Klenerman et al. [20] have presented a mathematical model to show that CTLs can markedly reduce the virus load by limiting virus production with minor effects on the half-life of infected cells.
For the beta-glucuronidase activity and the number of polymorphonuclear cells, the pattern of the effects was similar to the effects on the half-time tracer particle clearance.
Interestingly, NCD did not show significant effects on the half-duration of SWP (n = 5 Fig. 3, A, B, and G).
However, BV6 treatment had no detectable effects on the half-life of IRF1, which is known as an unstable protein because of its constitutive K48-linked polyubiquitination and subsequent proteasome-dependent degradation.
As unnecessarily conceded away goals go, it was not quite up there with John Arne Riise's own goal in stoppage time in last season's semi-final, but it still had a draining effect on the half time conversations, presumably within the home dressing room as well as without.
To test for genetic effects, environment effects, and genotype-by-environment-interaction effects on the smolts, one-half of the progeny from each cross group was reared in a traditional hatchery environment.
There was no effect of the half-sibfamily factor on these activities.
We showed that sterol depletion specifically reduced the half-life of the HMGCR13 transcript, but had no effect on the half-life of HMGCR13.
This is consistent with a previous report using C6 cells, which showed that hypoxia had no significant effect on the half-life of VEGFA mRNA until much later time points [38].
