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Since it is possible that effects on the clock countered effects on sleep, we measured the effect of shifting the clock multiple times over a 12 day period on energy stores (see Methods and Figure 1).
Together, these results indicate that MAN1 functions to promote BMAL1 expression, and thus exerting effects on the clock.
This work identifies ELF3 as repressing gene expression of clock components, resulting in widespread effects on the clock gene network.
Association with the PRR9 promoter provides a mechanism for ELF3's direct (PRR9) and indirect (CCA1/LHY) effects on the clock network.
This result indicates that the night-time light period has only minor effects on the clock, which is not re-entrained: if this were the case the peak of LHY expression observed with the night-time light period would not be expected to occur at around 24 hr after the previous dawn.
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To determine whether the effects of the clock on the light responses of A-type horizontal cells might result from changes in horizontal cell coupling, the extent of neurobiotin coupling was examined and found not to change under dark-adapted conditions during the day, night, subjective day and subjective night (Fig. 5).
Thus, it was concluded that the effect of the clock on checkpoint and apoptosis could be reliably compared only in the same cell strain in which the particular clock protein is downregulated by siRNA.
The effect of the clock jitter on the Analog-to-Digital Converter (ADC) performance is measured and presented.
The effect of the clock shift on glycogen varied among sexes and lines.
We saw little effect of the clock shift on triglycerides; as Figure 8B shows, only Oregon females exhibited significant changes over their control, increasing triglyceride stores.
It is clear from the figure that the bypassing has a dramatic effect on the clock cycles.
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