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The parameters that were found to have profound effects on the analyses include imperfections, boundary conditions and modelling assumptions.
Using different irradiances had only small effects on the analyses and the main conclusions of the study are unaffected by the type of illuminant used in the models.
Although this resulted in exclusion of 9% of the patients from our original study population, this probably had minimal effects on the analyses.
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On the other hand, these programmes will have little effect on the analyses because of the small number of working graduates.14.14
Adjusting for intragroup correlation had little effect on the analyses.
Importantly, adding these points has no effect on the analyses, and the original pattern of investment for small groups remains the same (in new Figure 7).
Clearly, exclusion of the distant taxa, Sordaria and Podospora, had a positive effect on the analyses, but only with this combination of methods.
This did not have any effect on the analyses of our results, as non-mating was randomly distributed across all mating pairs so that excluding them was not statistically biasing any combination (χ = 126; p = 0.32).
It is possible that these screens failed to identify all GroEL clients in the E. coli proteome; however, we do not think that a (presumably small) proportion of unclassified clients among our set of nonclient proteins will have a serious effect on the analyses reported below if anything, they ought to make the results more conservative.
Fifth, the data used in sample 3 (pilot test) of this study has a naturally occurring multi-level nature (individuals - units - nursing homes) which could have a biasing effect on the analyses reported here; the ICC(1) values for CRU scale score however revealed CRU is largely an individual concept in this dataset supporting our choice of analyses and limiting any potential bias in this regard.
Data from the first two day shifts were excluded in order to minimize practice effects on the tasks (analyses of the slope of the RT x set size function and accuracy in visual search indicated an improvement over the first two day shifts, but not from day 3 to day 4).
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