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In this report, we analyze the reciprocal effects of uptake sequence accumulation and selection on bacterial genomes and proteomes.
Two further analyses examined the within-gene effects of uptake sequences, asking first whether uptake sequences are more common in less conserved parts of proteins and then whether uptake sequences interfere with optimal coding and thus might reduce fitness.
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In Lineweaver-Burk plot analyses of mutual inhibitory effects on uptake of diphenhydramine and pyrilamine, the plots of diphenhydramine uptake in the presence and absence of pyrilamine intersected at the ordinate axis.
Comparing the results of different scenarios for vaccine uptake and temperature (Figure 3A,B) indicated that both vaccine uptake and temperature had a significant (P<0.001) impact on outbreak size, but that the effect of uptake depended on the temperature data-set (i.e. there was a significant (P = 0.006) interaction between uptake and temperature).
These results are in agreement with the predictions of Watanabe et al. for the effect of uptake transporter activity on plasma and liver concentration of pravastatin.
The EI, a normalized measure of sensitivity, for the effect of uptake transporter activity on muscle AUC exactly matched with that of the plasma AUC.
Cox proportional hazards regression models were used to examine the effect of uptake parameters as continuous variables, and to estimate hazard ratios (HRs).
Taken together, direct measurement of NA uptake and effect of uptake blockers, an age-related increase in stimulation-evoked fractional NA release in peripheral adrenergic nerves is not explained by an age-related change in the function of NA transporters.
To allow analysis of the effect of uptake sequence accumulation on the bacterial proteomes, we restricted our study to the perfect-consensus core 9-bp USS and 10-bp DUS sequences.
Rac1, Rho or Arf6 depletion did not have major effects on uptake of either probe (Figure 6A).
JNK inhibition had no effect on uptake of any strain or form of S. aureus.
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