Sentence examples for effects of this mutant from inspiring English sources
Hence, we speculated that the increase in the colony number in HeLa cells by ΔFHA was caused by the dominant-negative effects of this mutant upon the endogenous wild type CHFR protein [1].
The DN effects of this mutant LMP1 have been confirmed previously 12, 13.
In addition, it has been shown that POR A287P does not result in any inhibition of CYP19A1 activity (25), further highlighting the differential effects of this mutant.
This may reflect the apparently subtle effects of this mutant in vivo or a lack of sensitivity of the in vitro assay.
Unfortunately, Arboleda and colleagues did not take the opportunity to compare the effects of this mutant in vivo in Akt1-transfected or Akt3-transfected cells.
In fact, GKAP expression was increased by coexpression of C22S/C25S-TRIM3 compared to the negative control (empty vector), suggesting a dominant negative effect of this mutant.
Thus we mimic the effect of this mutant by increasing k2, the parameter that represents ROP activation in response to auxin.
In contrast, the AGS3 staining was often slightly reduced in cells expressing the mutant (Figs. 3E and 4C), a phenomenon presumably due to a dominant negative effect of this mutant on endogenous USP9x.
Interestingly, no significant effect of Y477F ezrin on the rate of primary growth of AC2M2 tumors was observed, thus mimicking the effect of this mutant on the growth behaviour of AC2M2 cells in 3D culture.
The increase in MF seen here following relatively short-term culture with a high level of expression of truncated MBD4 is demonstration in principle of the dominant negative effect of this mutant protein.
