Sentence examples for effects of these mutants from inspiring English sources
The effects of these mutants are more prominent, as compared to the counterion mutants (E165Q and D295N), although these mutants still retained the pH-dependency and showed small fractions of red-light sensitive components under the low pH conditions (Supplementary Figure 7d f).
Using circular dichroism, we find that 2 mutants affect the alpha-helical coiled-coil structure of individual molecules, and 2 mutants disrupt the lateral associations among individual molecules necessary to form higher-order assemblies, helping explain the dominant effects of these mutants.
This could account for the more pronounced effects of these mutants in cells bearing longer neurites (>50 µm) [10].
To further analyze the effects of these mutants overexpression in cell cycle, and being p27 mainly involved in G1/S transition, we studied more in details how T198 substitution affects this event.
Effects of these mutants on complex maturation were analyzed by Western blot of cell lysates and densitometry.
As can be seen, this reversed the inhibiting effects of the AS-mutants, suggesting that the effects of these mutants on proteasomal activity is via inhibition of S5a.
Although we cannot confirm that the Fbn2tm1Rmz, Fbn2fp-2J and Fbn2fp-3J alsoles also result in a homozygous phenotype of reduced strength, we feel it is highly likely given the likely effects of these mutant alleles on the Fbn2 protein and the similar digit fusion phenotype.
Whilst the cellular response to mutant matrilin-3 and COMP expression has been documented in detail (Bell et al., 2012; Leighton et al., 2007; Piróg-Garcia et al., 2007; Suleman et al., 2012), the effects of these mutant proteins on the organisation and composition of the cartilage ECM are not clearly defined.
The very poor fibrilization or the absence of fibrilization of the F3/A, W6/A and M10/A mutants prompted us to investigate the effect of these mutants in co-fibrilization assays.
As an in vivo model for studying the effect of these mutants after their extracellular application, we used the rat model of cerebral ischemia.
