After the mutational mapping, we performed in silico mutagenesis to observe the effects of the mutations on the interactions.
An obvious application of genetic knowledge about specific cancers is the development of drugs and antibodies that reverse the effects of the mutations in those cancers.
'The Race Is On' Then came the hard part: making a drug to create the effects of the mutations.
The observed effects of the mutations have been rationalized on the basis of an existing structural model of CPS1.
We mutated 10 buried residues in MBP to alanine and determined φc values from the effects of the mutations on global stability and proteolytic susceptibility.
The contributions of the nonconserved residues Leu54, Leu306, Leu308 and Phe311 to the binding were further investigated by site-directed mutagenesis, and the effects of the mutations on the Ki value were determined.
We characterized the effects of the mutations on the thermodynamic stability of scFvD1.3 by experiments of unfolding with urea, monitored by spectrofluorometry, and tested the additivity of their effects by double-mutant cycles.
The grafted loops do not strongly perturb the properties of the protein scaffold; however, specific effects of the mutations are observed for amino acids that are proximal in space to the sites of mutation.
The effects of the mutations are well-correlated among the three subunits.
The effects of the mutations when combined in the P2P3Shift mutant were not additive (Figure 6B).
To investigate if we could demonstrate such overt effects of the mutations, we turned towards the adult rescuants.
