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The effects of tSMS in Parkinson's disease remain unknown.
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The DNA extract used for testing the extent to which oligonucleotide spiking could improve the performance of tSMS was prepared from a horse fossil bone excavated at Thistle Creek in Pleistocene permafrost deposits.
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We subsequently used the mitochondrial information to assess further the quality of tSMS sequences.
A previous comparison of tSMS shotgun sequencing of modern versus ancient (Pleistocene horse bone) DNA extracts revealed no major difference in the base composition of the genomic region preceding sequencing reads [ 10].
Here, we aim at improving even further the performance of tSMS for ancient DNA by testing two novel tSMS template preparation methods for Pleistocene bone fossils, namely oligonucleotide spiking and treatment with DNA phosphatase.
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