In conclusion, monitoring of multiple microcolonies allows rapid determination of EMB susceptibility and has applications in diagnostics and for characterization of the inhibitory effect of EMB on strains carrying resistance associated mutations.
Addition of the EmbA, EmbB and EmbC inhibitor ethambutol (EMB), resulted in the loss of the upper band with only the lower band remaining, this being due to the noninhibitory effect of EMB on the terminal β(1 → 2) capping enzyme, AftB (Seidel et al. 2007; Figure 4, lane 3).
In addition to being investigated for effects of EMB, the data from experiment 2 were also analysed with respect to differences in constitutive transcript expression between strains.
To further investigate the effects of EMB exposure, a list of those features that were significantly affected by EMB treatment or for which significant interactions between treatment and other factors were observed (treatment × strain; treatment × time; treatment × strain × time) was compiled.
Probes with a significant main effect of EMB concentration were fold-change-filtered against the 0 ppb control (FC ≥ 1.5).
To analyse the effect of direct inhibition of Emb protein activity on pri-miRNA level, we treated 8 × 10 S2R+ cells with 25 ng/ml LMB (Sigma) or vehicle control (70% methanol) in 12-well plates for 2 h.
Our analysis offers a unique insight into the internal workings of a purportedly autonomous EMB and the partisan effects of automated redistricting on representation.
The effects of OMMT on the structure, glass transition, damping properties, thermal stability, mechanical properties and morphology of EMB were characterized by XRD, dynamic mechanical analysis, TGA, tensile test, and scanning electron microscopy.
† "Effects of Financial Globalization on Developing Countries".
Effects of fresh urine on USV induction.
