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The effects of different DNA damaging agents, as well as DDR kinetics have been well characterized in mammalian cells in vitro.
Here, we evaluated the potential of transient transfection in producing rFVIII in serum-free suspension HEK 293 cell cultures and investigated the effects of different DNA concentration (0.4, 0.6 and 0.8 μg/106 cells) and repeated transfections done at 34° and 37°C.
Even though the effects of different DNA damaging agents, as well as DDR kinetics, have been well characterized in mammalian cells in vitro, very little is so far known about the kinetics of DDR in tumor and normal tissues in vivo.
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The transcriptional process involves the combinatory effects of different DNA-binding proteins and histone modifications.
Fig. 2 Histogram of effect of different DNA concentration on peak current Fig. 3 The plot of reduction peak current of MB against log concentration of DNA. Figure 4 shows the results of the cross-reactivity study conducted with the portable DNA biosensor in the presence of various foodborne pathogens which mimicked the environment of this food sample.
We tested the effect of different DNA damaging agents on the abundance of TRIM29 mRNA.
More specifically, we investigated the effect of different DNA concentration and repeated transfection at 34° and 37°C on rFVIII production by human HEK293 cells cultured in serum-free suspension.
To investigate whether serum had an effect on delivery of different DNA molecules, complexes were incubated with the cells for 4 and 24 hours in the presence (10% serum) and absence of serum.
The error rate of different DNA polymerases has effects on the mutational rate of amplified large DNA fragments.
We further evaluated the effects of using different DNA amounts in the analysis.
These phenotypic data were used in this study to evaluate effects of different cytoplasmic DNA types.
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