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RT-PCR and Western blot analysis were used to determine the effects of CH on the expression of MDR1 mRNA and P-glycoprotein in K562/ADR cells when CH was used alone and combined with SP600125, a JNK inhibitor, to explore the effects of CH on JNK pathway.
To understand the effects of CH on dystrophin-deficient muscle in vivo, we exposed the Drosophila model for DMD (dmDys) to CH during a 16-day ascent to the summit of Mount Denali/McKinley (6194 meters above sea level).
The data showing the effects of Ch on cytoplasmic Ca2+ concentration (Fig. 6) support this sequence of events.
However, the effects of CH on changes of gene expression and muscle function in dystrophin-deficient states have not been fully characterized.
Western blot analysis was used to determine the effects of CH on c-Jun protein expression and phosphorylation, to explore the regulating effects of CH on c-Jun and phosphorylated c-Jun (p-c-Jun) proteins.
For the investigation of the effects of CH on JNK signal transduction pathway in protein level, protein extracts for gel-electrophoresis were made from the cells treated as above according to standard techniques.
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To quantify the effect of Ch on the other lipids, the average molecular area of an ideal mixture at 3 mN/m was calculated and compared to the observed molecular area.
The availability of the Drosophila model of DMD also allowed us to address the effect of CH on global gene expression and muscle function in the Drosophila DMD model.
To determine the effect of CH on dystrophin-deficient flies we screened the Affymetrix Drosophila Genome 2.0 GeneChip® platform and identified gene expression profiles of four individual CH-dmDys (Hypoxia protocol provided in Table S1) with four individual normoxic dmDys flies.
Supporting this contention, with this more detailed analysis, we were able to detect a significant effect of CH on both SV and HR.
To determine whether in vivo effects of PCB-77 are AhR-mediated, we defined effects of CH-223191, an AhR antagonist, on PCB-77 induced PCB-77 inducedglucose and impairmentlerance in LF-fed mice.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com