Sentence examples for effector dna from inspiring English sources

Furthermore, variation in base preferences of the individual structural repeats that make up the TAL effector DNA recognition domain makes targeting stringency tunable.

The principles that govern TAL effector DNA recognition make TAL effectors well suited for applications relevant to plant physiology and metabolism.

When TAL effector DNA binding domains targeting the ADH1 and TT4 loci were tested in both architectures, BamHI TALENs showed little in vivo activity.

As shown in Figure  3a, as low as 1 ng effector DNA induced GFP positive cells (white arrows), and the number of GFP positive cells increased in parallel with the increasing dosages of effector DNA to a maximum of 2.5 ug tested.

To directly test distal elements in a loss of function manner, we have fused the chromatin modifying enzyme lysine specific demethylase 1 (LSD1, also known as KDM1A) to transcription activator-like (TAL) effector DNA binding proteins.

Transcription activator-like effector nucleases (TALENs) are artificial proteins containing TAL effector DNA binding domains fused to the catalytic domain of the endonuclease, FokI (Christian et al., 2010; Miller et al., 2011).

In particular, hybrid recombinases consisting of catalytic domains from the resolvase/invertase family of serine recombinases fused to Cys2 His2 zinc-finger or TAL effector DNA-binding domains are capable of introducing targeted modifications into mammalian cells.

TALENs are artificially generated by fusing TAL effector DNA-binding domains to a Fok I nuclease domain, and successfully harnessed to custom-designed sequence-specific nucleases [ 15, 16].

Among epigenomic effectors, DNA methylation is believed to be a strong primary molecular mark having a major impact on intergenerational gene silencing [ 15, 16].

In plants, many protein families contain homologous proteins that have common biological functions but different specificities towards substrates, ligands, effectors, DNA, proteins and other interacting molecules, including other monomers of the same protein.

Because the computational tools available for TAL effector targets prediction do not yet support analyses of the citrus genomes [ 24, 25, 48], we designed a position weight matrix based on TAL effector-DNA association frequencies to predict and score EBEs for Xc and XaC TAL effectors in citrus gene promoters (Additional file 4).