We demonstrate the value and effectiveness of this assay with three protein targets: Akt2, ATF3, and NAEβ (the β-subunit of the neddylation activation enzyme).
Moreover, the results of the validation study comprising sensitivity, specificity, blind test, and casework with blood spots on filter paper and shed skin as a non-invasive sample confirmed the effectiveness of this assay in the forensic perspective.
As positive control to assess the effectiveness of the assay, the same aptamer, with both labeled and unlabeled, was used.
We also attempted to introduce a novel ligase-dependent probe production at equal or increased assay effectiveness, which would be less expensive than direct manufacture of full-length probes.
As shown in Figure 6A, MG132 treatment effectively increased the ubiquitinated C/EBPα protein which could not be detected in untreated cells, indicating the specificity and effectiveness of the assay system.
The instability of these antibodies further worsens the situation by reducing the effectiveness of the assay.
Despite its effectiveness, the DHS assay can vary significantly depending on the experimental parameters.
A larger study may be needed to assess the cost effectiveness of this assay compared to liquid culture.
In a second experiment we followed four wheat plants, biolistically transformed with the RNAi cassette, over up to four generations (T0 to T3) to investigate the effectiveness of our assay in selecting homozygous transgenic lines.
In the setting of a pharmaceutical programme, the cost effectiveness of every assay included in a testing regimen is evaluated continuously, and only those that contribute significantly to the drug development process are continued.
