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Our data showed that buoyant cyanobacteria could be flocked and effectively precipitated using a combination of PAC or chitosan with LMB or LRS.
To determine whether endogenous Abp1 and N-WASP associate in vivo, we carried out immunoprecipitations. Anti-Abp1 antibodies effectively precipitated Abp1 from rat brain extracts (Figure 4G).
Immunoprecipitation experiments were performed using NMMII isoform-specific antibodies, all of which (but not the IgG control) effectively precipitated designated myosin II isoforms from SK-CO15 cell lysates (Figure 2B).
By contrast, the native EGFR receptor is effectively precipitated by the GEP100 PH domain upon activation, i.e., when phosphorylated on pTyr1068 and/or pTyr1086.
We found that the selected antibody effectively precipitated cystatin C from human CSF, and that 1.6 μg of antibody (in 8 μL volume) was adequate to fully remove cystatin C from 18 μL of CSF (approximate 2 1 CSF to antibody volumetric ratio).
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We have previously reported that our anti-α6 antibody effectively precipitates striatal as well as recombinant α6β2 receptors expressed in HEK cells (Scholze et al., 2012).
Despite the widespread use of PTA to isolate PrPSc, the mechanisms by which PTA effectively precipitates PrPSc are still largely unknown.
In contrast to the mock-transfected sample, RBG-1 was effectively co-precipitated with the cytosolic region of SNT-1.
As shown in Figure 5C, ErbB1 was precipitated effectively by both full-length nucleolin and 212 C-terminal amino acids mutant, less effectively to GAR and RBD mutants, but not by the N-terminal mutant of nucleolin.
The microsegregation in single-crystal nickel-base superalloys, which may result in many undesired precipitated phases, can be effectively reduced by rapid solidification.
The chromium, nickel and zinc metals present in the precipitated solid volume were then effectively recovered.
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