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We estimated the highest effective duplication number of SDHB cDNAs during the two rounds of nested PCR in PBMCs using control plasmids containing the wild-type gene.
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On the basis of nucleotide misincorporation rates, enzymatic amplification of the wild-type plasmids for 38.5 effective duplications during nested PCR (above) are expected to generate random mutations in ∼0.15% of the PCR products within the 4 bp-long TaqI RE recognition site.
Requiring applicants to be electronically fingerprinted is a simple and effective way to prevent duplication and fraud by ensuring that people cannot be approved for multiple food-stamp benefits, as was too common in the years before welfare reform.
The Dossia group acknowledges as much, pointing out that "with employers paying almost half of all US healthcare costs, Dossia will be an important component in making the health-care system more efficient and effective, eliminating waste and duplication".Previous efforts have failed, but could Wal-Mart's rallying cry lead to an electronic revolution in health care?
Therefore, missing those articles hampers effective literature search needing duplication of efforts.
Our study aims at clarifying the effective contribution of gene duplication in the evolution of metabolic networks.
So, if protein connectivity is the most effective factor for the duplication of a gene, then the average paralog number of small complexes has to be greater than that of large complexes.
Arayici et al. (2012) argued that BIM implementation serves as a useful alternative to addressing key construction sector issues, and offer solutions to these in order to increase productivity, efficiency, quality; reduce costs, lead times and duplications, via effective collaboration and communication of stakeholders in remote construction projects.
In summary, OrthoMCL yields more, smaller protein groups than other methods, and is more effective in separating ancestral duplications (out-paralogs).
However, the most effective technique for finding duplications and further identifying new genes would be a genomic sequence comparison based on the availability of genome sequences.
Some 90% of single gene duplicates become silenced shortly after the duplication, leading to an effective rate g of duplications one order of magnitude lower, i.e., ~ 10-3 per million years [ 22, 25, 27, 28].
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