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Mutations disrupting the originally designated stem-loop IV did not exert any detectable effect on translation.
Our intention was to examine whether the effect on translation accuracy of adding synapomorphies to the ladders was similar before versus after instruction in phylogenetics.
The ribozyme was found to reduce the titer of infectious virus containing lacZ by 90 percent, but had no effect on translation of lacZ messenger RNA.
These results indicate that for students with stronger backgrounds in biology, the effect on translation accuracy of adding synapomorphies to the ladders is comparable to the effect of two days of instruction in phylogenetics.
All models have in common that miRNA binding has an inhibitory effect on translation initiation.
The mTOR inhibitor, rapamycin, had no inhibitory effect on translation of either type of mRNA under the same conditions.
The addition of 1 µM DCB-3503 produced about 50% inhibitory effect on translation of both non-polyadenylated and polyadenylated luciferase mRNAs (Fig. 5B).
We then analyzed the effect on translation of rare sequence variants in the RPS19 5'UTR found in subsets of DBA patients.
The finding that the first uORF has an opposite effect on translation efficiency implies that it may facilitate the reinitiation of translation in the downstream ORFs.
Consistent with this, hippuristanol appears to be well tolerated by cultured cells at the concentrations used, and has a specific and reversible effect on translation [21].
Lengthening the 5'UTR however, has a dramatic effect on translation initiation accuracy, eliminating translation from the downstream AUG and increasing translation efficiency.
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CEO of Professional Science Editing for Scientists @ prosciediting.com