Thus, replacing the side chain of Arg 517 with the methyl group of alanine has no significant effect on the primer strand, but uncouples the conformational transition of the template from that of the enzyme, thereby allowing the template strand to slip towards an active conformation even in the absence of the correct dNTP.
Mitochondrial uncoupling had the opposite effect on the PPP.
A similar positive effect on the ATP/ROS ratio was detected in adult flies, in which mitochondrial uncoupling in neurons was increased, and the life span extended.
On the other hand, Lis1 may uncouple ATP hydrolysis from linker docking at AAA5 through an allosteric effect on the ring.
This uncoupling of the guidance and the control problem is valid either when the torque commands have a negligible effect on the CoG motion or when the control time scale is much shorter than the guidance time scale.
In contrast, the distribution of membrane potential shifted from red (FL-2) to green (FL-1) fluorescence when carbonyl cyanide m-chlorophenylhydrazone (CCCP), a protonophore, was treated as a positive control, through its effects on mitochondrial uncoupling.
The reviewer's suggestion that the effects of nucleotide on the substrate be uncoupled from their effects on the protease, by measuring the cleavage of a short peptide corresponding in sequence to the interdomain linker of BiP is conceptually sound.
They argue that the uncoupling of motherhood and marriage has devastating effects on the kids, who, according to statistics, grow up poor, have behavior problems, drop out of school, have children out of wedlock themselves, and -- Yoffe left this last one out -- end up in prison.
In this study, we addressed this hypothesis by analyzing the effects of mitochondrial uncoupling on the pathways of intermediary metabolism in white adipocytes.
The effect of chemical uncoupling on lipid accumulation was assessed through biochemical assays and morphological analysis.
