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The salinity effect on the method sensitivity was investigated.
The species differences did not have a significant effect on the method.
The robustness of the method was tested at the VL level by introduction of four small but deliberate changes in the operating procedure and by the assessment of their effect on the method results.
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Hence the benefit of amenorrhoea appears to be independent of the effect of the method on estrogen-withdrawal.
However, we found that the benefit of amenorrhoea was independent of the effect of the method on ovulation.
The effect of the method of purification on the protein yield after hapten addition was also investigated.
We synthesize for the first time the knowledge on provider payment methods used in CBI in developing countries and the evidence on the effect of the methods on CBI performance.
Therefore, the effects of the method on the clinical interpretation of WR remain unclear, and it is uncertain which method would provide the more accurate and reproducible estimates.
It is found that these can have a significant effect on the methods considered.
Despite this, it is able to reduce its effect depending on the method adopted and the existing environment conditions.
Since the assay results of the antioxidant effect depend on the method used, a combined assay of several methods is required.
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