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Figure 2C shows that loss of Syt1 had no effect on the localisation of Arf3 in the presence or absence of Yel1.
It is noted that previous studies using the tagged vectors had shown that the reporter genes had no effect on the localisation and expression pattern of the ATXN7 protein [28].
We have deleted genes involved in the biogenesis of these lipids but found no effect on the localisation of DivIVA (Supplementary data).
In addition, expression of UAS-YFP-rab5 S43N had a similar, though more pronounced, effect on the localisation of βPS to loss of netrins or fra, with a reduction in the gradient and increased localisation to the PMEC/VM interface (Fig. 9G,H,K).
These findings indicate that although depletion of talin2 has no apparent effect on the localisation of talin1 in podosomes, or on the number of podosomes per cell, it does reduce the matrix-degrading capacity of macrophages without affecting the expression of MT1-MMP, MMP2 and MMP9.
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References. 1. Huber-Wagner S et al. Effect of the localisation of the CT scanner during trauma resuscitation on survival.
In the S-aggregates, the modelled microgravity conditions did not have any significant effects on the localisation of N-CAM and tyrosine hydroxylase, but they were shown to induce a switch of the GAP43 protein from the neurite-like processes to the cytoplasmic compartment.
Given the loss of RhoGEF2 and E-cad asymmetry upon removal of core pathway activity, we also examined the effects on the localisation of Zipper (MyoII), Bazooka and Armadillo (Arm), which are also asymmetrically distributed at these stages (Zallen and Wieschaus, 2004; Bertet et al., 2004; Blankenship et al., 2006).
By immunofluorescence staining and immune blotting, we found that HDAC inhibitors have no effect on the subcellular localisation and the expression levels of HDACs, suggesting that the regulation of subcellular localisation and the expression of HDACs are not involved in the antiapoptotic function of HDAC inhibitors (data not shown).
The treatment of U266 cells with bFGF had no effect on the subcellular localisation of FGFR3IIIc (results not shown).
In pigmented cells, treatment with 20(or)D3 or 1,25(OhadD3 had no effect on the intracellular localisation of p65 (data not shown).
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