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It shortened action potential duration (APD) in isolated rat ventricular myocytes, and increased the threshold current for triggering action potential, but had no effect on the inward rectifier K+ current IK1.
As shown in figure 3A, bath application of 1 mM 4-AP had no effect on the inward rectification or on the linear portion of the I/V curve but largely decreased the outward currents.
Nevertheless, IKss was distinct from IK1, because noradrenaline had no effect on the inward rectifier currents.
Blisterase had no discernable effect on the inward or outward voltage-gated currents in the cells measured.
Benzamil had no significant effect on the inward whole-cell current (carried mostly by bath K+ at -100 mV) (mean decreased insignificantly by 9.8%; from I-100 = -476 ± 112 pA to -429 ± 130 pA, n = 8).
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Furthermore, excluding phosphate (which is known to bind La3+) from the perfusion solution, and thus raising the concentration of free La3+, had no effect on the ischaemia-evoked inward current [the peak inward current was 3354 ± 582 pA (n = 11) in phosphate-containing solution and 2672 ± 427 pA (n = 5) in solution containing La3+ and no phosphate; P = 0.26].
Consistent with the results obtained for the corresponding residue (N342 in TM8) in GKPOT (Doki et al., 2013), Y388A had no detectable effect on the nitrate-induced inward currents, N-uptake, and sensor response, indicating the residue Y388 may not be involved in nitrate binding or transport cycle of CHL1.
Halothane was reported to be associated with AIVR due to a depressant effect on slow inward calcium current and intracellular calcium accumulation [4].
The traces in Figure 5A show the effect of increasing concentrations of Zn2+ on the inward currents elicited by applying ACh at EC20 to receptors with different stoichiometries.
If the country turned inward, the effect on the bottom-line of businesses from Unilever to General Motors would be huge.
In addition, CCBs show a chemosensitising effect (Helson, 1984) which is thought to be independent of effects on the classical slow inward calcium channel.
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