We showed that sterol depletion specifically reduced the half-life of the HMGCR13 transcript, but had no effect on the half-life of HMGCR13.
This is consistent with a previous report using C6 cells, which showed that hypoxia had no significant effect on the half-life of VEGFA mRNA until much later time points [38].
Depletion of p42 reversed this effect whereas depletion of p48 has no effect on the half-life of p85.
However, we did not find a pronounced effect on the half-life of the psd module under blue light (Table 1, Additional file 1: Figure S2 and S3).
Wild-type Qrr4 has a half-life of over 32 min, deletion of SL1 dramatically reduced the half-life to ∼5 min, deletion of SL2 and SL3 had little effect on the half-life, and deletion of both SL1 and SL3 also reduced the half-life to less than 5 min.
Figure 3B shows that deletion of either the gltIJKL locus or the sroC region alone dramatically increased the half-life of GcvB, from ∼1.7 min to > 15 min, whereas the GcvB target site in the 5′ UTR of gltI had little if any effect on the half-life of GcvB (see ΔCA mutant).
The half-life of Fc-fusions is typically shorter than intact Abs (1 2 weeks against 3 4 weeks; Suzuki et al, 2010), and so it might be expected that equivalent mutations would make a more significant effect on the half-lives and overall efficacy of Fc-fusions than mAbs.
Results show that the jet Reynolds number, the jet separation, the width-to-height ratio of jets, the depth between the front and back walls and the headspace above the injectors of the chamber have effects on the half deflecting oscillation.
