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We found that pooling all reads from various samples has a negative effect on sequence elongation.
Moreover, they found that the other structural measures had either no effect or no independent effect on sequence variability.
Therefore, we included all these factors in the analyses to factor out their potential effect on sequence evolution.
Another genomic determinant, the duplication span, is identified as exerting a significant negative effect on sequence rate asymmetry among paralogs.
I agree with the conclusion of the authors about biased HGT and its effect on sequence similarity.
It is therefore valid and useful approach to study its effect on sequence variability (see Results/Discussion, part 3.1, predictor 3).
Similar(52)
To test whether USP7 binding was responsible for the stimulatory effect on sequence-specific DNA binding by p53, we conducted EMSAs using another version of p53, p5382 360 (Figure 1A), which differs from p5382 393 in that it lacks the C-terminal regulatory region responsible for both USP7 binding [23] and nonspecific DNA binding.
Contaminants such as excess salts, PCR primers, and dNTPs have a detrimental effect on sequencing of PCR products.
Our observation that long-term storage of samples has no significant negative effect on sequencing results implies that it is possible to re-evaluate FFPE-samples from participants in previous clinical trials.
If the social request (i.e. the opened mouth) is responsible for increasing accuracy in sequence control because a social affordance is activated, we could find an effect on sequences directed to the conspecific even when they were unrelated to feeding.
Table 3 summarizes the effect on sequencing depth, more than 25 G b p of sequence data passed quality control.
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