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Importantly, treatments throughout the xenograft study had no significant effect on mouse weight (data not shown).
Cuprizone has a dose-dependent toxicity effect on mouse weight (Hiremath et al., 1998).
When mice reached 18 months of age, there was no longer an effect on mouse weight; however, the authors observed a positive dose response relationship between in utero PFOA exposure levels and abdominal brown AT mass but a negative relationship between PFOA and white AT mass.
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Several observations suggest that this is not the case: (1) there is no effect of the compound on mouse weight; (2) we observed that the effect of o-OPN on 275-3-2 tumours is lost under conditions of low-level light-at night conditions; and (3) there is no effect of o-OPN on the growth of an OPN-deficient tumour cell line (data not shown).
Fig. 4 a Effect of free and SMA Ral treatments compared to DSS on mouse weight.
No toxic side effects were observed on mice weight or biochemical parameters.
After treatment for 20 days, we found injection of DMSO or CCR2atg had little effect on mouse body weight.
These treatments had no significant effect on mouse body weight and the animals appeared healthy and active throughout the study.
All of the regimens tested were well tolerated by the mice, with no substantial effect on mouse body weight observed during treatment.
Successive injections of CnB at therapeutic doses for several months had no detrimental effect on mouse body weight and mouse liver and spleen indexes (data not shown).
These treatments had no significant effect on mouse body weight or toxicity in liver and the animals appeared healthy and active throughout the study (Additional file 1: Figure S1).
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