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Among the molding parameters studied, chemical foaming agent weight percentage has the most significant effect on cell size, cell density, and tensile strength.
On the other hand, results show that nanoclay has the least effect on cell size.
Results of Table 15 also state that nanoclay wt % has the least effect on cell size increase.
However, our results suggest that even strong TrpC3 knockdown has little effect on cell size while powerfully inhibiting hypertrophic gene expression.
Importantly, whereas TrpC3-knockdown strongly reduces gene expression associated with hypertrophy, it has a negligible effect on cell size and on myocyte beating.
Additionally, we measured both cell size and endogenous ANP expression simultaneously in the same PE-stimulated cells, and found that TrpC3-shRNA decreased ANP expression (Fig. 2F), but had no effect on cell size (Fig. 4D).
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Notably, the vacuolar morphology index robustly correlates with the auxin effect on cell sizes.
In fibroblasts, expression of Pax3 or Pax7, or dominant-negative inhibition of these factors, reproduce the effects on cell size, morphology and proliferation seen in myoblasts.
The effects on cell size were similar to those on organismal size and are displayed in Figure 2, the quantitative and statistical analysis of cell size phenotypes is shown in Figure 3.
Thus, loss of Akt (Fig. 5D) and Tor (Fig. 5G) results in a 27 30% increase in cell density, and expression of dominant negative versions of Dp110 (Fig. 5C), RagA (Fig. 6G) and dS6K (Fig. 6C) caused similar but weaker effects on cell size (7 14% increase in cell density; Fig. 6L and Table S1).
These data demonstrate that rapamycin's effects on cell size regulation are independent of the cell cycle.
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