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Fig. 11 Monitoring culture parameter effect on cell shape changes reviled by online flow cytometry and optic microscopy.
Taking into consideration the effect of Hcl1 on the growth and intracellular survival of mycobacteria, we decided to investigate whether Hcl1 has any effect on cell shape and morphology.
The other PMMs had no consistent effect on cell shape or actin filament distribution.
Instead, each of the isoforms has a distinct effect on cell shape, implying divergence of functions.
Taken together, we speculate that the effect on cell shape and motility induced by AFAP1L1 overexpression was not due to a modulation of Src activation.
These results prompted us to examine the possibility that AFAP1L1 exerts a regulatory effect on cell shape and motility together with a novel interacting partner.
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Because they form scaffolds that recruit and organize many other proteins, including those that regulate morphogenesis and cell cycle [5], it seems reasonable that changes in septins would have large effects on cell shape by virtue of their binding partners.
Another "Blue Box" mutant, Merlin BBA, has been shown to be tumorigenic in mammals [31], [32], though Merlin ΔBB and Merlin BBA are reported to show some differences with respect to effects on cell shape and adhesiveness [30], [31].
This protein has counter-adhesive properties, has effects on cell shape, immune surveillance and angiogenesis [ 5]; inhibits cell proliferation and delays cell cycle in the G1 phase [ 6].
Reducing the apparent density of Tol-OP-ST had significant effects on cell shape, morphology and numbers of vinculin-positive focal contacts.
We also included two closely related genes, FAM40A and FAM40B, which had only a small effect on migration in scratch wound assays, but which each had profound effects on cell shape: FAM40A depletion resulted in flatter cells, whereas FAM40B depletion increased elongation and is in the same PMM group as FMNL3, FNBP3 and LIMD1.
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