Further studies are ongoing to refute or corroborate the potentially dominant effect of this mutation.
To investigate the effect of this mutation in the fish, the authors used the CRISPR Cas gene-editing technique to generate zebrafish (Danio rerio) carrying mutated insra.
Previous research has shown that one effect of this mutation is to prevent the gene from interacting with the protein complex NCoR/HDAC3.
The strong protective effect of this mutation, which lies next to the aspartyl protease beta-site in APP, provides support for the hypothesis that reducing the beta-cleavage of APP may protect against Alzheimer's.
Assuming a recessive mode of inheritance, the effect of this mutation remains unclear, but is unlikely to provide a protective effect to the degree suggested by mouse knockout studies.
In an attempt to dissect the mechanism of escape of the Nef TW9 mutation, we investigated the effect of this mutation on CTL recognition of CD4+T cells infected with an engineered SIVmac239 that contained the TW9 mutation in Nef.
We also studied Hoxa-3 mutants, but found no effect of this mutation on the cad6 expression pattern.
We have examined the effect of this mutation on the structure and function of NCS-1.
However, the effect of this mutation is only intermediary as compared with AgmISG15 (see Figure 5b).
Based on molecular modeling, the effect of this mutation over protein stability is probably independent of p.D322G mutation found on the same allele.
The program replaces each of the interface residues with alanine residue and computes the effect of this mutation on the binding free energy of the complex.
