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In this study, we constructed a eukaryotic plasmid, pVAX-CDPK1, and examined the immunogenicity, and protective immune effect of this DNA vaccine in Kunming mice against T. gondii infection.
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The kinetics of the mucosal and systemic antibody secretion, patterns of antibody subtype production, cytokine production by the spleen, MLN lymphocyte, and protective effect of this DNA-prime/protein-boost regimen against T. spiralis infection were evaluated in mice in this study.
In this study, the effect of the DNA vaccine enhanced by α-GalCer was evaluated by immunizing BALB/c mice.
As for DNMT2, the absence of any effects of this DNMT on DNA methylation confirms previous literature data [ 25].
We sought to determine whether the property of caffeine to prevent P-TEFb LC release and prevents transcription inhibition could be due to a general protective effect of this drug in DNA damaged cells.
The effect of DNA extraction methods and DNA quality on the assessment of reference gene copy numbers was also investigated.
This effect of nontarget DNA arises from depleting the concentration of free PNA capable of interacting with DNA target due to adhesion of positively charged PNA molecules on the negatively charged DNA duplex.
This effect of free DNA originating from already adapted populations resembles the proposed benefit of transformation in using beneficial genes that the DNA pool retains from the past ('the genetic time travel effect') [ 32].
The effect of this activity on cellular DNA was measured in an assay based on the production of DNA-strand breaks.
Hence, based on previous reports (Thévenod and Friedmann 1999), we hypothesize that Cd-induced reduction of megalin and ClC5 mRNA levels is the consequence of an indirect effect of this heavy metal on DNA transcription involving ROS production and oxidative stress.
Due to the tethering effect of DNA looping this concentration can be much higher than the bulk free LacR concentration [2], [7], leading to increased occupancy of the primary site by the repressor and enhanced gene repression.
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CEO of Professional Science Editing for Scientists @ prosciediting.com