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One recurring motif involves juxtaposing quotations from Mr. Kim's film writings with scenes from North Korean movies that appear to illustrate them; the primary effect of these sequences is to make you wish you could see more of the North Korean films.
We are presently investigating the protective effect of these sequences against live influenza challenge.
The current names of pasteurellacean uptake signal sequences (USS) and neisseriacean DNA uptake sequences (DUS) could be misleading, as they do not accurately describe the effect of these sequences on DNA uptake and imply that they represent different genetic elements.
Manual evaluation of positively selected genes involved examination of the alignments, where in families with branches with Ka/Ks>1 and alignments where some sequences had a high percentage of gaps, the effect of these sequences on the alignment and subsequent calculation of Ka/Ks ratios was evaluated.
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We did identify base changes in several candidate genes, indicating a low rate of EMS effects on these sequences.
We will investigate the functional effect of these sequence changes in future work.
Both the TATAA box and the polyadenylation site for the D1-g gene were non-canonical sequences, however the effect of these sequence variations on expression is unknown.
No data were presented regarding the effect of these sequence changes on pathogenicity, and it is not known whether the original IgA antibody 412.67 is pathogenic in mice.
To investigate the effect of these sequence changes at K16 PtK2g418, PtK2 cells were transfected with plasmids expressing either wild-type K16, K16 p.Arg418Cys or K16 p.Arg418Pro cDNAs.
To investigate the effect of these sequence changes on mtDNA replication, we obtained the corresponding yeast mip1 alleles (Arg265Cys, Arg672Ter, Arg770Glu, Thr809Ter, and Val863Ala, respectively) and tested their effect on mitochondrial genome stability and replication fidelity.
To verify this, we executed permutation tests (see Methods), and found that even when the effect of these sequence features was subtracted from LD, the adjusted LD in conserved regions was still significantly weaker than that in non-conserved ones (p < 10-4).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com