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The fecundity data across four replicate populations were pooled after noting that there was no main effect of the replicate populations.
Moreover, here, the random effect of the replicate mating chamber was important, so all models had this random effect in addition to the random effect of female identity (nested within the identity of the replicate mating chamber).
The authors state that there was a significant effect of the replicate on Principal Component 2 (page 3, left column, third line from bottom), but later state that " sub-strain differences were comparable in the two replicates conducted one year apart" (page 5, left column, line 5 from top).
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Furthermore, by the same arguments as before, the sole effect of the replicated gene may be to increase α E by a factor of two (or N for an N-copy mutant).
By estimating the SNP effects of the replicated data sets, the standard deviation of the SNP effects is obtained.
Formula (1) and (2) were also used for the analysis of the greenhouse experiment where R i was the effect of the ith replicate.
Our study confirms an effect of the widely replicated TCF7L2 rs7903146 polymorphism on hyperglycemia in an adult Italian population-based cohort both in cross-sectional and longitudinal evaluation.
To investigate the effect of the number of replicates (r), we chose up to five replicates at each sampled time point.
For bias evaluation the final model was fitted to replicate datasets using the bootstrap resampling technique in Wings for NONMEM [35], and PK parameter estimates and random effects for each of the replicate datasets were obtained.
We explored the effect of the number of replicates on prediction accuracy using a RNA-Seq dataset [ 15] with 7 replicates for each of two conditions (control and E2-treated MCF-7 cells).
The effect of the rs2270915 was replicated in the second step population: AA homozygotes had a lower SBP than G carriers (137.4 ± 19.1 vs. 140.0 ± 20.2 mmHg, P = 0.004).
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