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The enhancing effect of the mutants was clearly IKKβ-dependent, as tested again in IKKβ-deficient 3T3 cells.
This must be an effect of the mutants because ectopic expression in wild-type flies allows translation and ectopic bristle formation.
We could also demonstrate an effect of the mutants miR-142-3p-m1 and -m2 on the 3′ UTR of the ZEB1 mRNA.
At 37°C the negative effect of the mutants on lysis rate might be explained by lower transcript levels for the lysis protein.
The conclusion in the text is that there is no effect of the mutants on activity, but an effect on telomere length.
As ZEB1 was not detectable with commercially available antibodies in the U2932 DLBCL cell line assayed, we did not further test the effect of the mutants on ZEB1 protein.
Similar(51)
The apoptotic-like effect of the mutant 373 was identical to that of MF37 but its necrotic-like activity was significantly reduced.
As the transferrin receptor is located correctly at the plasma membrane in TS-mutant cells at 42°C, impaired endocytosis is a direct consequence of the temperature-sensitive effect of the mutant clathrin.
The lifespan effect of the mutant depends on the presence of a functional TOR1 gene.
This might be the result of a cytotoxic effect of the mutant.
Numerical simulations of the effect of the mutant channel on sino-atrial (SA) node function then reproduced the SSS phenotype.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com