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In order to screen for genes which were inactivated by transposon insertion and those affected polarity effect of the inserted transposon, we introduced the Ω interposon cassette by replacing the gentamycin resistance gene (aacC1) of pTn Mod-OGm (Fig. 1).
The results were in agreement with those reported in this study thus indicating that the results were not influenced by a position effect of the inserted construct.
Thus, the present data cannot formally exclude a negative effect of the inserted sequence on 3xP3 function in non-drosophilids.
For all types except HPV-35 and HPV-18 the repressive effect of the inserted HPV sequence also decreased the translation (figure 4d).
The percentage of chimeric contigs between viruses and bacteria was insignificant, due to the low effect of the inserted prophages into the bacterial genomes to create chimeras.
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Effects of the inserted depth and diameter of fine particle outlet were investigated experimentally and numerically.
However, the markers that confer power are also potentially problematic as they can be cumbersome, risk phenotypic effects of the inserted genes, and accumulate as unwanted genes during successive mutagenesis cycles.
Thus, the phenotypes are considered to correctly reflect the effects of the inserted mutation.
A more plausible explanation for the differential effects of the inserted transgene among the four offspring pairs may be that positional effects caused the line-specific differences.
Effects of the inserted transgene differed among the four offspring pairs for the dependent variables powdery mildew resistance, ergot infection, seed number and seed yield as reflected in significant GM/control × offspring pair interactions (Figure 3B; powdery mildew infection: P = 0.022; ergot infection: P<0.001; seed number: P<0.001, seed yield: P<0.001).
To investigate the effects of the inserted 671-bp fragment on blaKPC-3 expression, plasmids pKPC3-184 pMU-KPC3-184 pMU-KPC3-184 pMU-KPC3-184g the vector pACYC184.
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