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To study the effect of the expression of the putative D-galacturonic acid transporter encoding gene, the D-galacturonic acid concentration in the medium was measured during growth.
To examine the effect of the expression construct VerUTR on 4T1 cells, we analyzed proliferation rates of the cells.
We also examined the effect of the expression of the different semaphorins on the adhesion of the various tumor cells to plastic or to fibronectin.
In experiments in which the effect of the expression of Sima was analyzed, cells were also transfected with the pAc5.1-Sima plasmid [31], or with an empty vector (pAc5.1-V5) that served as a negative control.
We compared the properties of human α3β4 neuronal nicotinic receptors expressed in Human embryonic kidney cells (HEK293) and in Xenopus oocytes, to examine the effect of the expression system and α∶β subunit ratio.
To investigate the effect of the expression of the above three c-CBL mutants on cell migration, we carried out wound healing assay as described in materials and methods.
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AM404 effect on extinction is independent of effects on the expression of conditioned fear, pain, locomotion, and baseline anxiety.
This, consequently, has a positive effect on the expression of rpoS, the master regulator of the general bacterial stress response.
In contrast, conditional deletion of Arid1a had variable, and often minimal, effect on the expression of these genes.
MK-2206 and cyclopamine that target other pathways had no effect on the expression of let-7i-5p (Fig. 5e, h).
ANP (10 -7) M) itself had no apparent effect on the expression of these protooncogenes.
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