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In the mitochondrial preparation, the predominant effect of SS on oxygen consumption rates (OCR) was to cause a decrease in maximal respiration.
Remarkably, in cells with a reduced content of HIPK2, the effect of SS on p21waf1,cip1,sdi1 expression was restored in the presence of Bleo as indicated by western blotting (Figure 4b, left) and densitometric analyses (right).
We began by investigating the effect of SS on ROS generation and observed that the absence of serum in culture medium for 4 h was enough to produce a significant increase in ROS levels.
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We investigated mechanisms of susceptibility during critical developmental periods to sidestream smoke (SS) exposure and evaluated the possible effects of SS on neural responses.
Our recent clinical practice of combining SSd administration with radiation in treating patients with hepatocellular carcinoma revealed that this joint treatment was more effective than either monotherapy alone, indicating a contributory effect of SSd on radiotherapy.
DOI: http://dx.doi.org/10.7554/eLife.06576.004 To identify the global effects of SS on brain activity, we compared the ALFF in the SS group with the Saline group 2 hr post-treatment using two-sample t-tests corrected for multiple comparisons.
However, the effect of SSd on tumors exposed to radiation is largely unknown.
This result may be attributed to the radiosensitization effect of SSd on hepatoma cells.
However, the mechanism underlying radiosensitization effect of SSd on HCC cells remains unclear.
To investigate the mechanism underlying the radiosensitization effect of SSd on HCC cells, we analyzed the changes in the cell cycle progression using flow cytometry after intervention.
The radiosensitizing effect of SSd on hepatoma cells is more obvious under hypoxia, and its mechanism may contribute to its effect on the G0/G1 and G2/M checkpoints of the cell cycle.
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